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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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TOPblunt PCR Cloning Kit（GTOP02） |
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TOPblunt PCR Cloning Kit
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TOPBlunt PCR Cloning Kit is suitable for cloning Pfu polymerase amplified PCR products with blunt ends at room temperature in 5min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of blunt-ended DNA fragments to the vector without using a regular ligase.
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《快速簡單》
TOPO cloning 系統
不需使用Ligase反應，僅需5分鐘即可完成 ligation反應。
不需額外做A-tailing
使用Ampicillin、藍白篩選。
《好用、高效率》
Insert 長度至少可達3 kb
載體帶有單一EcoR I切位, 容易轉殖 (subclone)。
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl) , 1 kb (50 ng/µl), 2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPBlunt PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen100 DNA Ladder
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TOPBlunt Vector Information
 
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| 1 vial of 20 μl |
TOPBlunt Vector (10 ng/μl) |
| 1 vial of 20 μl |
6X TOPBluntTM Buffer |
| 1 vial of 5 μl |
Control insert DNA (1 kb) |
| 21 vials of 100 μl |
DH5α Competent Cell |
| 1 vials 5 μl |
Control Plasmid |
| 1 vial of 400 μl |
M13 Control primer mix |
| 1 vial of 7.5 ml |
SOC medium |
| 1 vial of 1 ml |
Sterile water |
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| Products: |
Cat. No. |
Size |
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TOPBlunt PCR Cloning Kit
(Including competent cell)
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GTOP02-20 |
20 r
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TOPBlunt PCR Cloning Vector
(not including competent cell)
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GTOP02-V20 |
20 r
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