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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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pGM-T PCR Cloning Kit（GVT-202） |
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pGM-T PCR Cloning Kit
The pGM-T Vectors are linearized vectors with a single3´-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases.
The pGM-T Vectors are high-copy-number vectors containing T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of the enzyme β-galactosidase. Insertion a linactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates.
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《快速簡單》
載體帶有T7及SP6起動子, 可容易進行in vitro transcription 及sequencing 。
以X-Gal及IPTG (藍白篩選), 含insert插入者colonies呈現白色, 便於篩選。
使用Ampicillin篩選。
《好用、高效率》
選殖過程在1小時內完成。
載體帶有單一EcoR I切位, 容易轉殖 (subclone)。
《經濟方便》
使用者只要準備PCR產物即可, 不需額外準備其他材料, 減少額外花費。
直接選殖PCR產物 (0.1-4 kb) 至載體中。
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| 1 vial of 20 μl |
pGM-T Vector (50 ng/μl) |
| 1 vial of 01 μl |
T4 DNA ligase (3 U/μl) |
| 1 vial of 30 μl |
10X T4 DNA Ligation Buffer |
| 1 vial of 100 μl |
2X T4 Rapid Ligation Buffer |
| 1 vial of 5 μl |
Control Fragment (300 bp) |
| 21 vial of 100 μl |
DH5α Competent Cell |
| 1 vials 5 μl |
Control Plasmid |
| 1 vial of 400 μl |
Colony primer pairs |
| 1 vial of 15 ml |
SOC medium |
| 1 vial of 300 μl |
Nuclease-free water |
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| Products: |
Cat. No. |
Size |
| pGM-T PCR Cloning Kit |
GVT-202 |
20 r |
pGM-T PCR Cloning Kit (not including competent cell)
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GVT-202-0 |
20 r |
| pGM-T vector |
GVT-202-T1 |
20 r |
| GVT-202-T2 |
60 r |
| GM DNA Ligation Kit |
GVT-202-L
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20 r |
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