 |
Epigenetics |
 |
|
|
|
|
|
 |
| Epigenetics>【GeneMarkBio】 |
| |
|
| |
| |
Two-Step MMLV RT-PCR Kit（RP012-M） |
| |
|
|
Two-Step MMLV RT-PCR Kit
The GeneMark Two-Step MMLV RT-PCR Kit is provided with all components required to perform first-strand cDNA synthesis and second strand DNA amplification. SAMscript Reverse Transcriptase (SAMscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which is a frequent choice for complementary DNA (cDNA) synthesis because of its ease of use and low intrinsic RNase H activity. In the cDNA synthesis step, RNA was reverse transcribed by SAMscript RTase to produce its cDNA. For the subsequent amplification of the cDNA template, PCR Hot-Start Master Mix II is provided. It can eliminate amplification artifacts such as primer-dimer formation and mis- priming during preamplification stage and thus may provide improved specificity. PCR Hot-Start Master Mix II can be used for effective amplification up to 6 kb DNA.
產品敘述: 以RNA為模板，合成first strand cDNA，並利用Hot-start PCR mix來進行高效的2nd cDNA synthesis
酵素特性: 內含SAMscript reverse transcriptase及Hot-start PCR mix，具高專一性，此組試劑主要針對合成長片段cDNA所設計
引子種類: 產品內含Oligo (dT)18及random hexamer primer供您使用 (亦可使用自行設計的專一性引子)
應用範圍: 2nd cDNA synthesis, PCR, cloning等 |
|
|
Figure1. RT-PCR result of E.coli (DH5α) total RNA with amplification of second- strand cDNA fruK gene.
E.coli (DH5α) total RNA was reverse-transcripted into cDNA ,and fruK gene is further amplified using GeneMark Two-Step MMLV RT-PCR Kit.
M: GM100 DNA Marker
|
精品推荐：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
|
| |
|
|
| |
|
