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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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Midi Plant Genomic DNA Purification Kit（DP022MD） |
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Midi Plant Genomic DNA Purification Kit
The Midi Plant Genomic DNA Purification Kit provides a rapid, simple and effective approach to isolate the genomic DNA from plant tissue. The process is based on a modified salt precipitation method; the procedures involve cell lysis at 65°C, precipitation of proteins, polysaccharides and secondary metabolites, removal of cell debris by Filter paper, and DNA precipitation by isopropanol. Up to 1 g plant tissue can be processed and DNA purified with this kit is suitable for various applications, including PCR, restriction enzyme digestion, cloning, dot blot analysis, etc.
比傳統方式更快，完成只需2小時。
針對植物開發之產品: 適用各種植物、 真菌、中藥或感染植物之viral DNA等。
獨特配方可去除polysaccharides，增加DNA的純度。
Sample可達1 g，產量高，可達30~500 μg。
DNA純度高: 可直接cloning、PCR amplification、enzyme digestion、hybridization、sequencing等。
安全簡單: No phenol/chloroform，no spin column。
Purification of plant genomic DNA from rice leaves.
(1% of purified DNA were loaded on 1% agarose gel.)
Lane 1: 1 g of young rice leaf by Midi Plant Genomic DNA kit
Lane 2: 100 mg of young rice leaf by spin column kit from supplier A
Lane 3: 100 mg of young rice leaf by spin column kit from supplier B
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