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Epigenetics |
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| Epigenetics>【GeneMarkBio】 |
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Easy Tissue & Cell Genomic DNA Purification Kit（DP021E） |
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Easy Tissue & Cell Genomic DNA Purification Kit
The Easy Tissue & Cell Genomic DNA Purification Kit provides a simple and solution-based approach to isolate the genomic DNA from various animal tissues, culture cells and bacteria. The procedure involves cell lysis/proteinase K digestion, protein precipitation and DNA precipitation. Typical yield ranges from 30-80 μg. DNA purified with this kit is suitable for various applications, including PCR, and restriction enzyme digestion.
適用多種材料: bacterial (4 x 109), animal cells (1 x 107), tissues (25 mg), 昆蟲(25 mg), 線蟲 (25 mg), 老鼠尾巴 (1.5 cm), yeast (2 x 109)
過程不需spin column，過程簡單快速，只需20分鐘。
純化量多，可達 80 μg/reaction。
DNA純度高，可直接cloning、PCR amplification、enzyme digestion、hybridization、sequencing。
更安全: No phenol/chloroform。
Purification of zebrafish DNA by Easy Tissue & Cell Genomic DNA purification kit
1% of purified DNA were loaded on 1% agarose gel.
Lane 1: 15 mg of whole zebrafish
Lane 2: 8 mg of whole zebrafish
Lane 3: 5 mg of whole zebrafish
Lane 4: Tail fin of zebrafish
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