Plasmid Miniprep Purification Kit-II

The Plasmid Miniprep Purification Kit II is based on a very simple and rapid procedure of plasmid DNA from 1-10 ml liquid culture by spin column format. The kit includes two wash buffers to improve the quality of  plasmid DNA from both EndA⁺ and EndA^- E. coli strain. High quality DNA without RNA contamination, can be prepared in 10-15 min. The yield of DNA up to 40 μg is quantitative and reproducible, and the DNA is sufficient pure for direct use in any application, e.g. PCR, restriction digestion, cloning, automated DNA sequencing, southern blot, in vitro transcription assay, etc. 

產品特性 產量多: 每一反應可從1-10 ml culture 純化至多40 μg ultra pure plasmid DNA。 純度高: 純化完之DNA純度高, 可直接automatic sequencing, in vitro transcription, PCR, enzyme digestion 等試驗。 操作快速: 使用spin column方法，過程10～15分鐘。 獨特配方: 有效去除endonuclease，針對EndA+菌種BL21(DE3)、HB101和JM109特別有效。 實驗數據 2 ml Mini-preparation of pET29 recombinant in BL21 (DE3) by three different kits. After elution, add Hind III reaction buffer to final 1X concentration, incubation at 37℃ for 2 hours. M: Gen-kb ladder；1: Purified by GeneMark Plasmid Miniprep Purification Kit II；2: By competitor A；3: By competitor B 訂購資訊 Products: Cat. No. Size Plasmid Miniprep Purification Kit DP01 50 r DP01-300 300 r GM Plasmid Miniprep Kit DP01m 50 r DP01m-300 300 r Plasmid Miniprep Plus Purification Kit DP01-Plus 50 r DP01-Plus-300 300 r Plasmid Miniprep Purification Kit II DP012 50 r DP012-300 300 r Plasmid Midiprep Purification Kit DP01MD 20 r DP01MD-100 100 r Plasmid Midiprep Plus Purification Kit DP01MD-P10 10 r DP01MD-P50 50 r Plasmid Maxiprep Purification Kit (停產) DP01MX-10 10 r DP01MX-20 20 r Plasmid Maxiprep Plus Purification Kit DP01MX-P10 10 r DP01MX-P20 20 r

操作流程簡圖

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