The Quick-RNA™ MicroPrep kit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system withFast-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes.
The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the Zymo-Spin™Columns. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for enrichment of small and large RNAs in two separate fractions.
| Sample Sources |
Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™. |
| Storage |
Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification. |
| RNA Purity |
High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery |
Up to 10 µg RNA can be eluted into ≥6 µl RNase-free water allowing for a highly concentrated sample. |
| Sample Size |
Up to 106 cells or 5 mg tissue. |
| RNA Storage |
RNA is eluted with RNase-free water and can be stored frozen. RNase inhibitors can be included for prolonged storage. |
| Equipment Needed |
Microcentrifuge |
|
The Quick-RNA™ MicroPrep yields high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q & P but not with the Quick-RNA™ MicroPrep. Total RNA was isolated from human epithelial cells (sans DNase treatment).
|
RNA isolated with the Quick-RNA™ MicroPrepis DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.
|
High-quality total RNA (including small RNAs) from a wide range of samples – single to 106 cells.
Isolate small and large RNAs into separate fractions (optional).
DNA-free RNA for use in any downstream application. DNase I included.
| Quick-RNA™ MicroPrep |
50 Preps |
R1050 |
 |
| Quick-RNA™ MicroPrep |
200 Preps |
R1051 |
|
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