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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR Urine RNA Isolation Kit™---尿样RNA提取试剂盒
About ZR Urine RNA Isolation Kit™
ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe (not provided) and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated Urine RNA Buffer. The collected lysate can then be used immediately or at a later time following transportation and/or storage. Also, the Urine RNA Buffer is ideal for direct isolation of RNA from microvesicles that may be recovered from urine filtrates.
One-step RNA isolation occurs via matrix adsorption using Zymo-Spin™ IC Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit™ results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.
| Sample Sources |
Urine and other aqueous samples containing cells, biological sediment, microvesicle-associated RNA, etc. |
| RNA Purity |
High quality RNA is recovered in RNase-free water. Some DNA may be present in the final preparation of RNA. Complete removal of DNA can be accomplished by performing an in-column DNase I digestion. |
| RNA Recovery |
Typically, 0.2 to 3.0 µg RNA per 30 ml urine sample. The RNA binding capacity of the supplied columns is up to 10 µg. |
| Sample Size |
30 ml (standard reaction); can be increased/decreased proportionally. |
| RNA Storage |
RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is recommended for prolonged storage. |
| Equipment Needed |
Microcentrifuge; syringes (i.e., 30 ml, 1 ml). |
Quick, simple and reliable recovery of total RNA from cells, biological sediment in urine, large volume liquid samples and suitable for isolation of RNA from microvescicles.
Zymo-Spin™ column technology allows RNA to be eluted into volumes ≥6 µl and is ready for use in RT-PCR and other RNA-based procedures.
| ZR Urine RNA Isolation Kit™ |
20 Preps. |
R1038 |
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| ZR Urine RNA Isolation Kit™ |
50 Preps. |
R1039 |
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关于5-hmC
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