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Epigenetics |
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Epigenetics>【Zymo】 |
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Pinpoint™ Slide RNA Isolation System II(R1007) |
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Pinpoint™ Slide RNA Isolation System II
About Pinpoint™ Slide RNA Isolation System II
The Pinpoint™ Slide RNA Isolation System II is an innovative product for the isolation of RNA from any targeted area of fresh or paraffin-embedded tissue sectioned onto a glass slide. The system combines powerful Pinpoint™ tissue sampling methodology, a unique single-step RNA extraction/binding buffer, and Fast-Spin column purification technology to yield high quality RNA. Unlike current UV-based methods, this product makes isolation of tissue RNA simple and quick. No expensive specialized equipment is needed. Eluted RNA is well suited for subsequent RNA analyses including RT-PCR.
Format |
Spin Column. |
Processing Time |
5 hr |
Equipment |
Microcentrifuge |
Sample Sources |
Cells from tissue sections on glass slides. |
RNA Recovery |
Typically, up to 5 µg RNA is eluted into ≥ 8 µl RNase/DNase-free water allowing for a highly concentrated sample. |
RNA Storage |
Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C. |
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RT-PCR of RNA recovered from human tissue using the Pinpoint™ RNA Isolation System. Amplicons (in duplicate) are from A) a human ß-actin transcript; B) an arbitrary human transcript from Chromosome 3. M is 100 bp DNA Marker (Zymo Research).
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Allows for the isolation of total RNA from fresh and/or FFPE tissue sections.
Simple procedure combines Pinpoint™ tissue sampling technology with a one-step RNA extraction/purification method.
Omits the use of organic denaturants.
Product |
Size |
Catalog # |
How to Buy |
Pinpoint™ Slide RNA Isolation System II |
50 Preps. |
R1007 |
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表观遗传学产品全面解决方案列下:
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
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