Degradation and contamination of biological samples have been obstacles to scientific study, and may be particularly problematic in highly sensitive molecular-analysis techniques (e.g., PCR of low copy DNA). Use of cryogenic freezing methods for environmental/forensic sample preservation may often be too impractical to be employed. The Xpedition™ Fungal/Bacterial DNA MiniPrep, when used with a portable bead beating device [i.e., Xpedition™ Sample Processor (XSP), pg. 6], allows the researcher/investigator to "Take the Lab to the Field" and to the site of sample collection. The Xpedition™ Fungal/Bacterial DNA MiniPrep is designed for the simple, rapid isolation of DNA from tough-to-lyse fungi, including A. fumigates, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as from mycelium and Gram (+) and (-) bacteria. Samples from remote locations can be processed at the site of collection using the provided ZR BashingBead™ Lysis Tubes and a XSP (available separately, Cat. No. S6020, pg. 6). Sample processing occurs in a unique lysis/stabilization solution that effectively preserves DNA in fungal/bacterial lysates for subsequent storage and transport. Later, when convenient with the researcher, the kit's Fast-Spin column technologies are used for the isolation of inhibitor-free DNA from the lysates. Eluted DNA is ideal for PCR for various genotypic analyses including metagenomics, barcoding, phylogenetics, forensics, etc. An overview of the procedure is shown below.
| Format |
Bead Beating, Spin Column Purification |
| DNA Recovery |
Typically, up to 25 µg total DNA is eluted into 100 µl (25 µl minimum) DNA Elution Buffer per sample. |
| Equipment |
Microcentrifuge, Vortex, Portable Disruptor/Bead Beater (optional) |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
50-100 mg (wet weight) fungi or bacteria or up to 200 mg tissue. This equates to approximately 109 bacterial cells, 108 yeast cells and 107 mammalian cells. Spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| DNA Purity |
High quality, polyphenolic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 <1.8 |
"Take the Lab to the Field" and to the site of sample collection with state of the art Xpedition™ Sample Prep and DNA preservation technologies.
For efficient BashingBead™ lysis and isolation of inhibitor-free, PCR-quality DNA from tough-to-lyse fungi (e.g, yeast) and bacteria.
Unique lysis/stabilization solution keeps DNA intact in lysates for storage and transport following remote sample processing with the Xpedition™ Sample Processor (XSP).
| Product |
Size |
Catalog # |
How to Buy |
| Xpedition™ Fungal/Bacterial DNA MiniPrep |
50 Preps. |
D6206 |
 |
