The Pico Methyl-Seq™ Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.
| Sample Sources |
The protocol is designed for 10 pg – 100 ng genomic DNA input. DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator™ (Cat. No. D4010). |
| Equipment Needed |
Microcentrifuge, thermo-cycler |
| Sequencing Platform Compatibility |
This system is compatible with Illumina’s TruSeq chemistries for the HiSeq™ and MiSeq™ sequencing platforms. |
Agilent 2200 TapeStation D1K gel of libraries prepared (from B1-G1) using 10 pg, 20 pg, 100 pg, 1 ng, 10 ng, and 100 ng, respectively.
