| DNA Recovery |
Typically, up to 5 µg total DNA (per well) can be eluted into as little as 10 µl of low salt DNA Elution Buffer or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%. |
| DNA Size Limits |
From ~50 bp to 23 kb. |
| Sample Sources |
Any step in a standard ChIP protocol including:
a) Samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical or enzymatically-mediated DNA shearing.
b) Reverse cross-linked samples eluted from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS.
Note: This kit can also be used for DNA purification from PCR, restriction digests, kinase, phosphatase and other enzymatic reactions. |
| DNA Purity |
High-quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS. |
Rapid high throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K digested samples, PCRs, and other enzymatic reactions.
Plate design permits DNA elution at high concentrations into minimal volumes (≥10 µl/well).
Omits the use of organic solvents and the need for ethanol precipitation.
Eluted DNA is well suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.
| ZR-96 ChIP DNA Clean & Concentrator™ |
2 x 96 Preps |
D5206 |
 |
| ZR-96 ChIP DNA Clean & Concentrator™ |
4 x 96 Preps |
D5207 |
|
