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Epigenetics |
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Methylated-DNA IP Kit---DNA甲基化免疫自沉淀试剂盒
About Methylated-DNA IP Kit
The Methylated-DNA IP Kit utilizes immunoprecipitation technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the capture and separation of methylated DNA from non-methylated DNA in only a few hours (see figure below). Typically, over a hundred-fold enrichment of methylated DNA vs. non-methylated DNA can be achieved with the use of this kit. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. The product is provided with control DNA and primers for use as a control.
| Format |
Magnetic Beads |
| Processing Time |
3 hrs |
| Applications |
Utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA with a highly specific anti-5-methylcytosine monoclonal antibody for the "capture" and separation of methylated DNA from non-methylated DNA in only a few hours, from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. |
| Components |
Comes as a two component kit: the kit box along with a separate icebox containing the cold-storage components. |
| Enrichment Factor |
> 100 fold for Methylated versus Non-methylated DNA. |
| Optimal DNA Input |
50 - 500 ng |
Methylated DNA enrichment for large-scale DNA methylation analysis.
Includes a highly specific anti-5-methylcytosine monoclonal antibody for defined, reproducible results.
Includes control DNA and primers for easy monitoring of the entire procedure.
Eluted, ultra-pure DNA is ideal for use in subsequent molecular based analyses (e.g., assembling genomic libraries and determining genome-wide methylation status).
| Product |
Size |
Catalog # |
How to Buy |
| Methylated-DNA IP Kit |
10 Rxns. |
D5101 |
 |
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表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
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