EZ-96 DNA Methylation-Lightning™ Kit
About EZ-96 DNA Methylation-Lightning™ Kit
The EZ-96 DNA Methylation-Lightning™ Kit features high-throughput (96-well) bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
| DNA Recovery |
> 80% |
| Conversion Efficiency |
>99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines. |
| Optimal DNA Input |
Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
DNA sequencing results following bisulfite treatment. DNA with methylated C at nucleotide position #5 was processed using the EZ DNA Methylation™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remains intact while the unmethylated cytosines at positions #7, 9, 11, 14 and 15 are completely converted into uracil following bisulfite treatment (detected as thymine following PCR).
Methylation Plot From Reduced Representation Bisulfite Sequencing (RRBS). Data shows the relative percentage of methylation at individual CpG sites in mouse DNA. Methylation percentage is shown across a ~3 Mb region of mouse chromosome 19. Bisulfite sequencing libraries were prepared using mouse genomic DNA prepped with the Genomic Clean & Concentrator™ (D4010, D4011 – Zymo Research) and bisulfite converted using EZ DNA Methylation™ technology prior to Next-Gen sequencing.
Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
| EZ-96 DNA Methylation-Lightning™ Kit (Shallow-Well) |
2x96 rxns |
D5032 |
 |
| EZ-96 DNA Methylation-Lightning™ Kit (Deep-Well) |
2x96 rxns |
D5033 |
|
精品推荐：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
| RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶， |
