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Epigenetics |
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| Epigenetics>【Zymo】 |
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EZ-96 DNA Methylation-Gold™ Kit(D5007) |
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EZ-96 DNA Methylation-Gold™ Kit---EZ DNA甲基化试剂盒-GOLD
About EZ-96 DNA Methylation-Gold™ Kit
The EZ-96 DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
| Format |
96-Well |
| DNA Recovery |
> 75% |
| Processing Time |
3 hrs |
| Equipment |
Centrifuge with microplate carrier |
| Binding Capacity |
5 µg/well |
| Elution Volume |
Shallow-well: ≥ 30 µl, Deep-well: ≥ 15 µl |
| Conversion Efficiency |
> 99% |
| Input |
Plasmid, genomic, and endonuclease digested DNAs. |
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DNA sequencing results after bisulfite treatment.DNA with methylated CmpG (at nucleotide position #5) was processed using the EZ DNA Methylation-Gold™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remained intact while the unmethylated cytosines (i.e., positions #7, 9, 11, 14 and 15) were completely converted into uracil following bisulfite treatment and detected as thymine following PCR.
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High-throughput (96-well) bisulfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
Desulfonation and recovery of bisulfite-treated DNA with a 96-well plate.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
| EZ-96 DNA Methylation-Gold™ Kit (Shallow Well Format) |
2x96 Rxns |
D5007 |
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| EZ-96 DNA Methylation-Gold™ Kit (Deep Well Format) |
2x96 Rxns |
D5008 |
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