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Epigenetics |
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| Epigenetics>【Zymo】 |
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EZ DNA Methylation™ Kit---EZ DNA甲基化试剂盒
About EZ DNA Methylation™ Kit
The EZ DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product's innovative in-column desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. This kit is validated for use with Illumina'sGoldenGate® and Infinium® Assays.
| Format |
Spin Column |
| DNA Recovery |
> 80% |
| Processing Time |
12 - 16 hrs |
| Equipment |
Microcentrifuge |
| Binding Capacity |
5 µg/prep |
| Elution Volume |
≥ 10 µl |
| Conversion Efficiency |
> 99% |
| Input |
Plasmid, genomic, and endonuclease digested DNAs. |
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Conversion of non-methylated cytosine. A 459 bp PCR product amplified from either DNA converted with the EZ DNA Methylation™ Kit (lane 1) or from untreated DNA (lane 2) was digested with EcoR I. The cytosine at the EcoR I site was converted to uracil during treatment, preventing cleavage of the DNA by the endonuclease.
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Streamlined, proven procedure for bisulfite conversion of DNA.
Desulphonation and recovery of bisulfite-treated DNA with a spin column.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
| EZ DNA Methylation™ Kit |
50 Rxns |
D5001 |
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| EZ DNA Methylation™ Kit |
200 Rxns |
D5002 |
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表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这 |
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