Select-a-Size DNA Clean & Concentrator™
About Select-a-Size DNA Clean & Concentrator™
The Select-a-Size DNA Clean & Concentrator™Kit (Select-a-Size DCC™) provides the quickest and easiest method for purifying a desired range of DNA fragments sizes from library preps, PCR, endonuclease digestions, ligations, etc. Simply adjust the binding conditions for the desired cutoff, bind, wash, and elute. Selectively recover ≥300 bp, ≥200 bp, ≥150 bp, ≥100 bp, ≥50 bp DNA fragments or perform a double size selection. Unique Zymo-Spin column technology yields high-quality DNA in just minutes that is suitable for next generation sequencing, PCR, and other downstream applications. The entire purification procedure can be performed in as little as 7 minutes for 2 preps or 20 minutes for 24 samples. (See figures below).
| Processing Time |
7 minutes |
| DNA Size Limits |
From ~50 bp to 23 kb. |
| Sample Sources |
DNA from PCR, library preparation, sonication or any other enzymatic reaction |
| DNA Purity |
Eluted DNA is of high quality and well suited for ligations, restriction endonuclease digestions, library preparation cleanup, and next generation sequencing. |
| Optimal DNA Input |
up to 3 µg |
| Equipment Needed |
Microcentrifuge. |
| DNA Cutoffs |
Single size selection: ≥50 bp, ≥100 bp, ≥150 bp, ≥200 bp, ≥300 bp
Double size selection: >700 bp |
Select-a-Size DNA Clean and Concentrator allows for selection at ≥300 bp, ≥200 bp, ≥150 bp, ≥100 bp and ≥50 bp. DNA was size selected according to the Select-a-Size DNA Clean and Concentrator protocol and the results were analyzed by Bioanalyzer. 700 ng of sonicated salmon sperm DNA was used as a standard input to evaluate size selection efficiency and cutoff. Eluted DNA was diluted 1:20 prior to being loaded on the High Sensitivity DNA Chip for analysis.
Select-a-Size DCC can be used for double size selection of samples in ranges from 50-700, 100-700, 150-700, and 200-700. The desired DNA range was selected according to the Select-a-Size DNA Clean and Concentrator protocol and the results were analyzed by Bioanalyzer. 700 ng of sonicated salmon sperm DNA, and a 70 bp amplicon was used as a standard input to evaluate size selection efficiency and cutoff. Eluted DNA was diluted 1:20 before loading onto the Bioanalyzer High Sensitivity DNA Chip for analysis .
Quick and easy 7 minute spin-column procedure to select for ≥300 bp, ≥200 bp, ≥150 bp, ≥100 bp, ≥50 bp DNA fragments or perform a double size selection.
Clean and concentrate DNA from enzymatic reactions and library preparations in as little as 10 µl of nuclease free water
Eluted DNA is well suited for use in next generation sequencing, PCR, DNA ligation, endonuclease digestion, etc.
| Product |
Size |
Catalog # |
How to Buy |
| Select-a-Size DNA Clean & Concentrator™ |
25 Preps |
D4080 |
 |
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