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Quick-DNA™ Universal 96 Kit(D4070) |
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Quick-DNA™ Universal 96 Kit
About Quick-DNA™ Universal 96 Kit
The Quick-DNA™ Universal 96 Kit is the easiest method for high-throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-SpinTMtechnologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 15 µl. Zymo-Spin™ Plates ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
| Equipment |
Water bath or heat block (55oC), microcentrifuge, and vortex. |
| Sample Sources |
See full protocol for details |
| DNA Purity |
High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0). |
| Elution Volume |
DNA can be eluted into as little as 15 µl DNA Elution Buffer or water. |
| DNA Yield |
The DNA binding capacity of each well is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled. |
| DNA Size |
Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| DNA Applications |
DNA isolated using the Quick-DNA™ Universal 96 Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications. |
| DNA Types |
The Quick-DNA™ Universal 96 Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. Not recommended for small cell-free DNA isolation from urine, serum, and plasma. |
| Workflow Overview |
Utilize a Proteinase K Digestion and Zymo-Spin™ Column for effective recovery of DNA. See page 5 for more information. |
High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ Universal Kit. DNA purified using the Quick-DNA™ Universal Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
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HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ Universal Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ Universal Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.
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Easy and reliable high-throughput DNA purification from any sample source (whole blood, biological fluids, cultured/monolayer cells, solid tissues, etc.).
Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA-sequencing, arrays, and methylation analysis.
| Quick-DNA™ Universal 96 Kit |
2x96 Wells |
D4070 |
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| Quick-DNA™ Universal 96 Kit |
4x96 Wells |
D4071 |
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