Quick-DNA™ Universal Kit
About Quick-DNA™ Universal Kit
The Quick-DNA™ Universal Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin™Column technologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 35 µl. Zymo-Spin™ Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
| Equipment |
Water bath or heat block (55oC), microcentrifuge, and vortex. |
| DNA Purity |
High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0). |
| Elution Volume |
DNA can be eluted into as little as 35 µl DNA Elution Buffer or water. |
| DNA Yield |
The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled. |
| DNA Size |
Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. |
| DNA Applications |
DNA isolated using the Quick-DNA™ Universal Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications. |
| DNA Types |
The Quick-DNA™ Universal Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. Not recommended for small cell-free DNA isolation from urine, serum, and plasma. |
| Workflow Overview |
Utilize a Proteinase K Digestion and Zymo-Spin™ Column for effective recovery of DNA. |
High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ Universal Kit. DNA purified using the Quick-DNA™ Universal Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
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DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA™ Universal Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed.
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HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ Universal Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ Universal Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.
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Extract high-quality DNA easily and reliably from any sample source (biological fluids, cultured/monolayer cells, solid tissues, etc.).
Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
| Quick-DNA™ Universal Kit |
50 Preps |
D4068 |
 |
| Quick-DNA™ Universal Kit |
200 Preps |
D4069 |
|
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