Genomic DNA Clean & Concentrator™-25
About Genomic DNA Clean & Concentrator™-25
The Genomic DNA Clean & Concentrator™-25 (DCC™) is for quick (5 minute) recovery of ultra-pure, large-sized DNA(e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Bufferto a sample and then transfer the mixture to the supplied Zymo-Spin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
| DNA Recovery |
Typically, up to 25 µg total DNA per column can be eluted into ≥35 µl of low salt DNA Elution Buffer or water. |
| DNA Size Limits |
Capable of purifying small DNA fragments > 50 bp and large sized DNAs up to 200 kb. |
| Sample Sources |
DNA from impure preparations of genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and whole genome amplified (wga) DNA. Can also be used for the purification of low molecular weight DNA (50 bp to 10 kb) from PCR, endonuclease digestion, post-RT cDNA synthesis, etc.. |
| DNA Purity |
High-quality (A(260/280) ≥ 1.8) high molecular weight DNA ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS. |
Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from anyenzymatic reaction or impure preparation (e.g., Proteinase K digestion). No messy precipitations!
Unique spin column for low volume (≥35 µl) elution of ultra-pure, high-yield DNA.
Eluted DNA is ideal for PCR, restriction endonuclease digestion, Sanger and Next-Gen sequencing, etc
| Genomic DNA Clean & Concentrator™-25 |
25 Preps |
D4064 |
 |
| Genomic DNA Clean & Concentrator™-25 |
100 Preps |
D4065 |
|
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