The Oligo Clean & Concentrator™ provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure.
There is no need for organic denaturants or chloroform. Instead, the kit features Fast Spin column technology and employs a single-buffer system that allows for efficient DNA/RNA adsorption to the matrix of Zymo-Spin™ IC Column. DNA/RNA is washed and concentrated into a small volume of water (≥6 µl). Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
| Sample Sources |
Enzymatic reaction mixtures containing DNA/RNA fragments (radioactive-, biotin-, DIG-labeled) and oligonucleotides ≥16 nt. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| Recovery Volume |
Binding capacity of the Zymo-Spin™ IC column is 10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery >90 %. The column can be eluted with ≥6 µl. |
| Compatibility |
Single-straded (ss) and double-stranded (ds) DNA and RNA. |
| Purity |
High-quality DNA/RNA (A260/A280 nm <1.8; A260/A230 nm <1.8) eluted with water is especially well suited for hybridization, sequencing, ligation, and PCR. |
| Size Limit |
For oligonucletides ≥16 nt, up to 23 kb. |
The Oligo Clean & Concentrator™ facilitates >90% recovery of ssDNA oligonucleotides (A) and efficient short oligo and nucleotide removal (B).
Quick (2 minute) recovery of ultra-pure DNA and RNA oligonucleotides
Complete removal of dyes, salts, enzymes, nucleotides, and short oligos.
≥6 µl elution with zero retention Fast Spin columns
Eluted DNA/RNA is well suited for use in hybridization, sequencing, PCR, ligation, etc
| Oligo Clean & Concentrator™ |
50 Preps. |
D4060 |
 |
| Oligo Clean & Concentrator™ |
200 Preps. |
D4061 |
|
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