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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR Plasmid Gigaprep Kit(D4056) |
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ZR Plasmid Gigaprep Kit
About ZR Plasmid Gigaprep Kit
2 - 10 mg of high quality, endotoxin free (for transfection) plasmid in less than one hour.
The ZR Plasmid Gigaprep Kit employs a modified alkaline lysis method in conjunction with DNA binding beads (ZymoBeads™) to isolate high quality endotoxin-free (for transfection) plasmid DNA in less than an hour. The purified DNA is suitable for use in transfection, restriction endonuclease digestion, ligation, bacterial transformation, PCR amplification, DNA sequencing and other sensitive molecular biology applications. The innovative patented colored buffers included in the kit permit error-free visualization of both complete bacterial cell lysis and neutralization. Additionally, the uniquely designed Midi Filter allows the capture of ZymoBeads™ either by centrifugation or vacuum. The unique design of the filter also allows for low elution volumes of 2-3 ml directly in supplied Elution buffer or water, thus eliminating the need for plasmid DNA precipitation and resuspension steps common to other column-based gigaprep procedures. The ZR Plasmid Gigaprep Kit is designed for use with a combination of both centrifuge, and vacuum manifold, or a centrifuge alone, therefore providing flexibility in large scale plasmid DNA purification from E. coli. An overview of the purification procedure is shown below
| Format |
Spin Column with Affinity Beads |
| DNA Purity |
Plasmid DNA is endotoxin free (for transfection) and is well suited for transfection, ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical Abs260/280 index is ≥ 1. |
| Elution Volume |
≥ 3 ml |
| Plasmid DNA Size |
Up to 25 kb |
| Plasmid DNA Yield |
≥ 2 mg per preparation (1000 ml culture), depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. (Can be scaled up to 10 mg by increasing culture volume and the amount of Zymobeads used.) |
| Procedure |
Performed at room temperature (15-30°C) using a vacuum manifold and/or a centrifuge. |
Innovative chemistry and streamlined procedure for consistent high concentration plasmid recovery directly in water or low salt buffer.
Colored buffers for visualization of complete bacterial cell lysis and neutralization.
| ZR Plasmid Gigaprep Kit™ |
5 Preps. |
D4056 |
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| ZR Plasmid Gigaprep Kit™ |
10 Preps. |
D4057 |
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