The ZR BAC DNA Miniprep Kit is for the efficient isolation of BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
| Format |
Spin Column |
| Processing Time |
15 min |
| DNA Purity |
High-purity and endotoxin-free (<50 EU/μg) plasmid DNA in low salt buffer or water; typical A260/280≥ 1.8. Eluted DNA is suitable for sequencing, endonuclease digestion, PCR, and other reactions requiring highly purified DNA. |
| Plasmid DNA Size |
Up to ~200 kb. |
| Plasmid DNA Yield |
Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed,and culture growth conditions. The typical yield is 1 μg per 3 ml of culture. |
| Procedure |
Can be conducted at room temperature, between 15-30°C. |
| Recovery Volume |
≥ 10 µl |
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HindIII and NotI digestion of BAC DNA. A BAC (~160 kb) from a RPCI-11 human BAC library (CHORI) was purified from DH10B cells (Invitrogen) using the ZR BAC DNA Miniprep Kit. Digestion with NotI removed the ~148 kb insert from the 11.6 kb pBACe3.6 cloning vector 1 (?). M: 1 kb DNA ladder (Zymo Research).
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For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps.
Unique column design: zero buffer retention and low-volume (≥ 10 µl) elution.
| ZR BAC DNA Miniprep Kit |
25 Preps. |
D4048 |
 |
| ZR BAC DNA Miniprep Kit |
100 Preps. |
D4049 |
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