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Epigenetics |
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| Epigenetics>【Zymo】 |
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Zymoclean™ Large Fragment DNA Recovery Kit---凝胶大片断DNA回收试剂盒
About Zymoclean™ Large Fragment DNA Recovery Kit
The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to 10 μg total DNA per column can be eluted into ≥ 10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥ 23 kb, the recovery is 50-70%. |
| Equipment |
Microcentrifuge |
| DNA Size Limits |
50 bp to ~200 kb |
| Sample Sources |
DNA excised from TAE and TBE-buffered agarose gels. |
| DNA Purity |
High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
Recovery of large DNA fragments. The Zymoclean™ Large Fragment DNA Recovery Kit was used to recover λ DNA digested with HindIII and separated by agarose gel electrophoresis. Lane C: λ-HindIII digest; lanes 1 & 3: recovered 23 kb λ-HindIII fragments; lanes 2 & 4: recovered 9 kb λ-HindIII fragments. Lane λ: intact λ phage DNA; lanes 5, 6: intact λ ~48 kb bands.
Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
Unique column design for low volume (≥ 10 μl) elution of ultra-pure, high-yield DNA.
Eluted DNA is well suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.
| Zymoclean™ Large Fragment DNA Recovery Kit |
25 Preps |
D4045 |
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| Zymoclean™ Large Fragment DNA Recovery Kit |
100 Preps |
D4046 |
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