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Epigenetics |
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| Epigenetics>【Zymo】 |
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Zyppy™-96 Plasmid Miniprep |
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Zyppy™-96 Plasmid Miniprep
About Zyppy™-96 Plasmid Miniprep
The Zyppy™-96 Plasmid Miniprep is the fastest and simplest high-throughput method available for efficient isolation of plasmid DNA from E. coli. The kit features a modified, Pellet-Free alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization/Clearing Buffer. The remaining DNA purification steps are straightforward and simple. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.
| Format |
96-well |
| Processing Time |
45 min |
| DNA Purity |
Eluted plasmid DNA is well suited for ligation, sequencing, restriction endonuclease digestion, transfection, in vitro transcription, and other sensitive applications requiring pure DNA. Abs260/280 is ≥1.8 |
| Plasmid DNA Size |
Up to 25 kb |
| Plasmid DNA Yield |
Up to 10 µg per preparation, depending on the plasmid copy number, culture growth conditions, and the strain of E. Coli processed. |
| Procedure |
Performed at room temperature (15-30°C) with a centrifuge with micro plate carriers. |
| Recovery Volume |
≥ 30 µl per well |
The fastest and simplest high-throughput procedures for purifying high-quality plasmid DNA.
Innovative pellet-free procedures and in-situ lysis omits cell pelleting and resuspension steps.
Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
| Zyppy™-96 Plasmid Miniprep |
2x96 Preps. |
D4041 |
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| Zyppy™-96 Plasmid Miniprep |
4x96 Preps |
D4042 |
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| Zyppy™-96 Plasmid Miniprep |
8x96 Preps |
D4043 |
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