The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product featuresFast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
| Format |
Spin Column |
| DNA Recovery |
Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment |
Microcentrifuge |
| DNA Size Limits |
50 bp to 23 kb |
| Sample Sources |
DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| DNA Purity |
High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
 |
The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.
|
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
| DNA Clean & Concentrator™- 25 - Uncapped Columns |
50 Preps |
D4005 |
 |
| DNA Clean & Concentrator™- 25 - Uncapped Columns |
200 Preps |
D4006 |
|
| DNA Clean & Concentrator™- 25 - Capped Columns |
50 Preps |
D4033 |
|
| DNA Clean & Concentrator™- 25 - Capped Columns |
200 Preps |
D4034 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
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