The ZR-96 DNA Clean & Concentrator™-5 products provide purification of up to 5 µg DNA per well from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. The product facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
| Format |
96-well Plate |
| DNA Recovery |
Typically, ≤ 5 µg total DNA (per well) can be eluted with ≥ 10 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment |
Centrifuge with microplate carriers |
| DNA Size Limits |
50 bp to 23 kb |
| Sample Sources |
DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| DNA Purity |
Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| RNA Purity |
Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
 |
|
Clean & Concentrated DNA. DNA samples, such as the PCR products shown here, can be efficiently purified and concentrated using the DNA Clean & Concentrator™-5
|
 |
| |
 |
 |
| Kit / Catalog # |
ZR-96 DNA Clean-up
Kit (D4017 and D4018) |
ZR-96 DNA Clean &
Concentrator™-5
(D4023 and D4024) |
| Binding Plate |
Silicon-A™ Plate |
Zymo-Spin™ I-96 Plate |
| Style |
Shallow-Well |
Deep-Well |
| Height of Binding Plate |
19 mm (0.75 inches) |
35 mm (1.38 inches) |
| Binding Plate/Collection Plate Assembly |
43 mm (1.69 inches) |
60 mm (2.36 inches) |
| Binding Cap./Minimum Elution Volume |
5 µg/30 µl |
5 µg/15 µl |
Clean and concentrate up to 5 μg DNA (per well) with ≥ 10 μl elution volume in as little as 15 minutes with 0 μl wash residue carryover.
Deep-well filtration plate designs allow DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
| ZR-96 DNA Clean & Concentrator™-5 - Deep-well |
2x96 Preps. |
D4023 |
 |
| ZR-96 DNA Clean & Concentrator™-5 - Deep-well |
4x96 Preps |
D4024 |
|
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