The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spincolumn technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

DNA yield from Zyppy™ Plasmid Miniprep Kit and a kit from Supplier A. Plasmid DNA (pGEM®) was digested with EcoRI prior to agarose gel electrophoresis. Performed in triplicate. M, ZR 1 kb DNA Marker (Zymo Research)