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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR-96 DNA Clean-up Kit™ (Shallow well format) |
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ZR-96 DNA Clean-up Kit™ (Shallow well format)
About ZR-96 DNA Clean-up Kit™ (Shallow well format)
The ZR-96 DNA Clean-up Kit™ provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A™ Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.
| Format |
96-well Plate |
| DNA Recovery |
Typically, up to 5 µg total DNA (per well) can be eluted into as little as 30 - 40 µl water per sample. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| Equipment |
Centrifuge (with microplate carriers) |
| DNA Size Limits |
75 bp-23 kb |
| Sample Sources |
DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| DNA Purity |
Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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High-throughput DNA processing. Crude preparations of a 3 kb plasmid DNA from bacterial lysates were processed using the ZR-96 DNA Clean-up Kit™. Following elution from the plate, 48 samples were then separated in a 0.8% (w/v) agarose gel.
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| Kit / Catalog # |
ZR-96 DNA Clean-up
Kit (D4017 and D4018) |
ZR-96 DNA Clean &
Concentrator™-5
(D4023 and D4024) |
| Binding Plate |
Silicon-A™ Plate |
Zymo-Spin™ I-96 Plate |
| Style |
Shallow-Well |
Deep-Well |
| Height of Binding Plate |
19 mm (0.75 inches) |
35 mm (1.38 inches) |
| Binding Plate/Collection Plate Assembly |
43 mm (1.69 inches) |
60 mm (2.36 inches) |
| Binding Cap./Minimum Elution Volume |
5 µg/30 µl |
5 µg/15 µl |
Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. ZR-96 Silicon-A Plate™ design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
| ZR-96 DNA Clean-up Kit™ |
2x96 Preps. |
D4017 |
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| ZR-96 DNA Clean-up Kit™ |
4x96 Preps. |
D4018 |
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