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Epigenetics |
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| Epigenetics>【Zymo】 |
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Quick-gDNA™ Blood MidiPrep |
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Quick-gDNA™ Blood MidiPrep
About Quick-gDNA™ Blood MidiPrep
The Quick-gDNA™ Blood MidiPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.
| Format |
Spin Column |
| DNA Recovery |
Up to 125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. |
| Processing Time |
20 min |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
Up to 3 ml (see protocol) whole blood, plasma, or serum from humans, mice, rats, etc. |
| DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8 |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| Equipment Needed |
Centrifuge or vacuum source and manifold, microcentrifuge, vortex |
Quick method for the purification of high quality DNA from up to 3 ml whole blood, plasma, and serum, in less than 20 minutes using innovative Fast-Spin column technology. Up to 125 µg/prep..
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
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