Quick-gDNA™ Blood MicroPrep
| Format |
Spin Column |
| DNA Recovery |
Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. |
| Processing Time |
15 min |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
Sources: blood, plasma, or serum from humans, mice, rats, etc. |
| DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8 |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| Equipment Needed |
Microcentrifuge |
The Quick-gDNA™ Blood MicroPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.
Quick purification of high quality DNA from whole blood, plasma, and serum in less than 15 minutes using innovative Fast-Spin column technology.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| Quick-gDNA™ Blood MicroPrep |
50 Preps. |
D3070 |
 |
| Quick-gDNA™ Blood MicroPrep |
200 Preps. |
D3071 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。
