About Quick-DNA™ Urine Kit

The Quick-DNA™ Urine Kit is an innovative product designed for easy, reliable, and rapid isolation of cellular and/or cell-free DNA from up to 40 ml of urine. The product features a uniquely formulated urine DNA stabilization reagent that also functions as a precipitation reagent. After collection, total (cellular and cell-free) or cell-free urine can be stored at ambient temperature for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The DNA is subsequently purified and concentrated using Zymo-Spin™ IC-S Columns. Urine DNA isolated with the Quick-DNA™ Urine Kit is ideal for qPCR, array, methylation analysis, and other downstream applications.

DNA Quality	High quality DNA can be used for downstream application, such as PCR, bisulfite treatment/methylation detection, array, etc.
Sample Sources	Urine.
DNA Yield	The DNA binding capacity of the column is 5 μg. Note that DNA yield may vary depending on the urine itself. Female urine typically yields more DNA than male urine. Urine DNA from healthy female individual ranges on average from 6 - 1000 ng/ml. DNA from healthy male individual ranges on average from 2 - 20 ng/ml.
Equipment Needed	A centrifuge, a microcentrifuge, and a heat block / water bath.
Recommended Sample Volume	Up to 40 ml of urine per standard preparation. The sample volume can be scaled up or down if required.
DNA Size	Capable of recovering DNA fragments from 100 bp to 23 kb.

DNA yields increase linearly with increasing volumes of urine from healthy subjects extracted using theQuick-DNA™ Urine Kit. DNA was isolated from 1 ml, 10 ml, 25 ml, and 40 ml urine. DNA concentration was quantified using the Femto™ Human DNA Quantification Kit (Zymo Research, E2005).	Both cellular and cell-free DNA can be effectively purified from urine using the Quick-DNA™ Urine Kit. 5 ml of urine from a healthy female donor was processed and DNA was eluted in 20 μl final volume. 1 μl of the sample was analyzed using a 2200 Tapestation.

 

Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.

Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to 1 month at ambient temperature

Zymo-Spin™ column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA sequencing, arrays, and DNA methylation analysis.

Product	Size	Catalog #	How to Buy
Quick-DNA™ Urine Kit	50 Preps.	D3061