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Epigenetics |
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| Epigenetics>【Zymo】 |
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ZR Genomic DNA™ -Tissue MiniPrep---基因组组织DNA小量提取试剂盒
About ZR Genomic DNA™ -Tissue MiniPrep
The ZR Genomic DNA™-Tissue MiniPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, parasitic, microbial, viral) from a variety of solid tissues. The product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and are also compatible with inputs including: buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K then add the Genomic Lysis Buffer, vortex, and transfer the mixture to the supplied spin column. PCR inhibitors are effectively removed during the purification process and purified DNA is suitable for downstream applications including: PCR, Southern blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, etc.
| Format |
Spin Column |
| Processing Time |
25 min |
| Sample Sources |
Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit. |
| DNA Purity |
High quality DNA for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc., is eluted with DNA Elution Buffer or water (A260/A280 ≥1.8). |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| DNA Yield |
The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1 - 3 µg DNA per mg skeletal, heart, and brain tissues and 3 - 5 µg DNA per mg liver, kidney and lung tissues. Human whole blood will yield 3 - 7 µg DNA per 100 µl blood sampled. DNA is eluted into ≥ 50 µl DNA Elution Buffer or water. |
| Equipment Needed |
Water bath or heat block (55°C), microcentrifuge, and vortex. |
| DNA Size |
Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, Parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb - 35 kb. |
For high quality DNA purification from solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources
Combines Proteinase K digestion with innovative Fast-Spin column purification technology.
Isolated DNA is ideal for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| ZR Genomic DNA™-Tissue MiniPrep |
50 Preps. |
D3050 |
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| ZR Genomic DNA™-Tissue MiniPrep |
200 Preps. |
D3051 |
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