| |
|
| |
|
ZR-96 Quick-gDNA™---快速-基因组96孔DNA提取试剂盒
About ZR-96 Quick-gDNA™
The ZR-96 Quick-gDNA™ features a simple, high throughput (96-well) procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.
For processing, simply add the specially formulated Genomic Lysis Buffer to the samples, vortex, and transfer the mixtures to the wells of the supplied Silicon-A™ Plate. There is no need for organic denaturants or Proteinase K digestion because of the unique chemistries featured in the kit. Instead, the product yields high-quality, purified DNA in just minutes (see below). PCR inhibitors are effectively removed during the purification process. DNA purified using the ZR-96 Quick-gDNA™ is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
| DNA Recovery |
Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. |
| Equipment |
microcentrifuge, vortex, centrifuge w/ microplate carriers |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered |
| Sample Sources |
Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed. |
| DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
| Workflow Overview |
Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
|
Genomic DNA isolated from mouse tail snips using the ZR-96 Quick-gDNA™. A total of 30 mouse tail snips were homogenized with Zymo Research's Squisher-8™ then processed using the ZR-96 Quick-gDNA™. About one third of the number of eluted DNAs was then separated in a 0.8% w/v agarose gel (shown in lanes 1 to 30).
|
Quick, high throughput (96-well) purification of DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 25 minutes.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| ZR-96 Quick-gDNA™ |
2 x 96 Preps. |
D3010 |
 |
| ZR-96 Quick-gDNA™ |
4 x 96 Preps. |
D3011 |
|
| ZR-96 Quick-gDNA™ |
10 x 96 Preps. |
D3012 |
|
精品推荐：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
| RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 总体DNA甲基化极易定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1030-48 |
|
| 总体DNA甲基化极易定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1030-96 |
|
| 总体DNA羟甲基化极易定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1032-48 |
|
| 总体DNA羟甲基化极易定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1032-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
|
|
|
| |
|
