 |
Epigenetics |
 |
|
|
|
|
|
 |
| Epigenetics>【Zymo】 |
| |
|
| |
| |
Zymoprep™ 酵母质粒抽提试剂盒I（D2001） |
| |
|
|
Zymoprep™ Yeast Plasmid Miniprep I---Zymoprep™ 酵母质粒抽提试剂盒I
About Zymoprep™ Yeast Plasmid Miniprep I
The Zymoprep™ is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but usingZymolyase™ as the first solution. There is no need for glass beads, phenol, or vortexing. Instead, plasmid DNA is reliably recovered from yeast cells whether colonies, patches on plates, or liquid cultures are sampled. Plasmid yields are typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures. This kit also works well with low copy number yeast plasmids. Recovered plasmid DNA is in TE buffer and can be used for E. coli transformation, Western blotting, PCR, etc.
| Format |
Isopropanol Precipitation |
| Elution Volume |
≥ 35 µl |
| Plasmid DNA Size |
DNA up to 23 kb. |
| Compatibility |
S. cerevisiae, C. albicans and S. pombe, and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase™). |
Simple procedures for plasmid rescue from yeast.
Ideal for low copy and hard-to-isolate plasmids.
For isolation of plasmid DNA for downstream applications such as PCR, transformation, hybridization, etc.
| Product |
Size |
Catalog # |
How to Buy |
| Zymoprep™ Yeast Plasmid Miniprep I |
100 Preps. |
D2001 |
 |
|
| |
|
|
| |
|
