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Epigenetics |
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| Epigenetics>【Zymo】 |
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dNTP Mix
About dNTP Mix
dNTP Mix and dATP, dTTP, dGTP, dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
| Reagent |
Volume |
Final Concentration |
| 2X Reaction Buffer |
25 µl |
1X |
| dNTP Mix |
0.5 µl |
0.25 mM each dNTP |
| Forward Primer (10 µM) |
Variable |
0.3 to 1 µM |
| Reverse Primer (10 µM) |
Variable |
0.3 to 1 µM |
| Template DNA |
Variable |
< 200 ng/50 µl |
| ZymoTaq™ DNA Polymerase (5 U/µl) |
0.4 µl |
2 U/50 µl |
| ddH20 |
to 50 µl |
- |
| Total Volume |
50 µl |
|
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| Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations. |
| Concentration |
dNTP Mix: 5 µmol/500 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and dCTP).
dATP, dTTP, dGTP, dCTP: 25µmol/250 µl as a [100 mM] solution. |
| Storage |
dNTP Mix and dATP, dTTP, dGTP, dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C. |
Ready to use dNTP Mix (dATP, dTTP, dGTP, dCTP) of ultra high purity; >99% trisphosphate by HPLC
Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities
| dNTP Mix |
10 mM |
D1000 |
 |
| dATP |
100 mM |
D1005 |
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| dTTP |
100 mM |
D1010 |
|
| dGTP |
100 mM |
D1015 |
|
| dCTP |
100 mM |
D1020 |
|
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