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Epigenetics |
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Epigenetics>【Zymo】 |
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Zymo-Spin™ IV-HRC
About Zymo-Spin™ IV-HRC
The Zymo-Spin™ IV-HRC is a durable polypropylene Fast-Spin filtration column filled with a unique matrix that features a unique snap off base and sealable green screw cap. It is ideal for removing PCR/RT inhibitors including polyphenols, humic acids and fulvic acids from DNA/RNA preparations derived from water or soil microbes. The column filtration membrane has an approximate 10 - 20 µm pore size. Capacity is 50 - 200 µl.
Format |
DNA/RNA inhibitor removal filtration column |
Compatibility |
Microcentrifuge, Vacuum Manifolds |
Matrix / Construction |
Silica-based with 10-20 µm pore size / Polypropylene, snap off base, sealable screw cap |
Volumetric Capacity |
50 - 200 µl |
Zymo-Spin™ IV-HRC |
50 Pack |
C1010-50 |
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Collection Tubes (2 ml) |
50 Pack |
C1001-50 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |
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