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  新品---尿液样本 m6A 定量检测试剂盒(比色法)(A-P-9015)
   

尿液样本 m6A 定量检测试剂盒(比色法)                                                     货号:A-P-9015

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

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背景资料:Nucleobase m6A, a modified form of adenosine converted by adenosine methyltransferases is widespread in different cellular RNAs and also found in DNA. The biological importance of DNA/RNA m6A-methylation as a major epigenetic modification in phenotype and gene expression has been recognized widely. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In humans, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. Urinary excretion of m6A is an indication of the whole body turnover or the degradation of DNA/RNA, especially tRNA. The urinary m6A level can be changed with a change of the bodies’ turnover of m6A DNA/RNA or alteration of cellular DNA/RNA m6A status. A number of studies have indicated that m6A excreted in urine has the potential to act as a cancer biomarker. For example, an elevated level of urinary m6A was observed in colorectal cancer patients with active disease states

产品描述:In this ELISA-like inhibitory competitive immunoassay, urine samples and the m6A standard are first incubated with a m6A antibody solution and then transferred to strip wells coated with m6A polynucleotide. The well is washed to remove any unbound reagents after incubation and then a detection antibody is added to generate a signal that can be measured colorimetrically by reading the absorbance in a microplate spectrophotometer. Because m6A in the urine sample inhibits the binding of m6A antibody to m6A coated on the well, higher concentrations of m6A in the urine sample lead to a reduced binding of the antibody to the m6A on the well. Therefore the signal or OD intensity measured from the well will be inversely proportional to the amount of m6A in the urine sample and the amount of m6A in the urine sample can be quantified by a comparison with a predetermined m6A standard

产品特点:

Innovative colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours

96 strip-well microplate format makes the assay flexible: manual or high throughput analysis

3.Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent

4.The level of m6A measured in human urine samples using this kit is comparable to that detected by HPLC method

5.A novel assay principle allows high sensitivity to be achieved. The detection limit can be as low as 0.01 ng/assay well

6.Low input range of urine for each assay with a volume of 1 to 20 µl and an optimal volume of 5 µl

7.Optimized antibody and enhancer solutions allow for high specificity to m6A, without cross-reactivity to unmethylated adenosine

8.Negative control and positive standard are included, which are suitable for quantification of m6A in free form and m6A contained in DNA/RNA fragments from different urine samples

数据分析: 

操作流程 标准曲线 柱状图分析
尿液样本 m6A 定量检测试剂盒(比色法)流程 将m6A标准品以不同浓度添加到孔中,使用尿液样本 m6A定量检测试剂盒(比色法)来测吸光值

使用尿液样本 m6A定量检测试剂盒(比色法)精确定量来自人类尿液样本不同体积的m6A 水平。结果与MS-LC一致。甚至更好。

 


参考文献:

1.Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。

定购信息:    
产品名称 规格 操作手册 货号 询价
尿液样本 m6A 定量检测试剂盒(比色法) 96次 英文PDF-中文PDF A-P-9015-96 点击这里给我发消息
尿液样本 m6A 定量检测试剂盒(比色法) 48次 英文PDF-中文PDF A-P-9015-48  
总体RNA甲基化极易定量检测试剂盒(荧光法) 96次 英文PDF-中文PDF A-P-9009-96 点击这里给我发消息
总体RNA甲基化极易定量检测试剂盒(荧光法) 48次 英文PDF-中文PDF A-P-9009-48  
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) 48次 PDF A-P-4059-48  
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) 96次 PDF A-P-4059-96  
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 12次 PDF A-P-1056A-12
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 24次 PDF A-P-1056A-24 点击这里给我发消息
96孔DNA甲基化极速修饰试剂盒(磁珠法)  96次 英文PDF-中文PDF A-P-1050-096  
96孔DNA甲基化极速修饰试剂盒(磁珠法)  192次 英文PDF-中文PDF A-P-1050-192  
抗人C5a/C5a desArg单克隆抗体(克隆号2942) 100ug C-HM2078  
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 100ug C-HM2022F  
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 50ug C-HM2008  
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 50ug C-HM2006  
抗小鼠TREM-2单克隆抗体(克隆号6E9) 100ug PDF  C-HM1129F 点击这里给我发消息
m6A RNA甲基化定量检测试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-9005-96  
RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-P-9003-050  
EZ RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-R5001  

表观遗传学产品全面解决方案列下:   

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1036-96  
DNA羟甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1037-96  
DNA甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1034-96  
总体DNA甲基化极易定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1030-48 点击这里给我发消息
总体DNA甲基化极易定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1030-96  
总体DNA羟甲基化极易定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1032-48 点击这里给我发消息
总体DNA羟甲基化极易定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1032-96  
总DNA甲基化定量试剂盒 48 次 英文PDF--中文PDF A-P-1014B-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 英文PDF--中文PDF A-P-1014B-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别

时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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