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| 精彩产品>免疫生物学...E... |
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人结肠癌细胞核提取物 货号：E-36035
Caco-2 nuclear extract
背景资料：Caco-2 nuclear extract was prepared from cell cultures of the human colorectal adenocarcinoma Caco-2 cell line. The Caco-2 cell line was originally derived from a colon carcinoma and functionally demonstrates properties resembling enterocytes of the small intestine. When confluent, these cells form a polarized epithelial monolayer with properties similar to those of the mucosal lining of the small intestine. In research, Caco-2 cells are most commonly as in vitro models of the small intestinal mucosa to study the physiological and biochemical properties of intestinal permeability, adsorption, transport and metabolism of ions, small molecules, and drug therapies.
产品描述：
Caco-2 nuclear extract is specifically recommended for analysis of the biophysical and biochemical properties associated with small intestinal mucosa.
产品应用：
Caco-2 cells are grown to 90% confluence before harvesting' the nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
产品使用：
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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