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  Magna ChIP 染色质免疫沉淀A/G试剂盒(A-17-10085)
   

Magna ChIP 染色质免疫沉淀A/G试剂盒                                                     货号:A-17-10085

Magna ChIP™ A/G Chromatin Immunoprecipitation Kit

背景资料:Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes

产品描述:Unlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously

产品特点:

Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day

Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone

3.Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions

4.Complete kit with all required reagents for reliable and reproducible results

5.Compatible with ChIPAb+ validated antibody and primer sets

6.Magna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack;

7.Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type)

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。

定购信息:    
产品名称 规格 操作手册 货号 询价
Magna ChIP 染色质免疫沉淀A/G试剂盒 22次 PDF A-17-10085 点击这里给我发消息
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 12次 PDF A-P-1056A-12  
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 24次 PDF A-P-1056A-24  
96孔DNA甲基化极速修饰试剂盒(磁珠法)  96次 英文PDF-中文PDF A-P-1050-096  
96孔DNA甲基化极速修饰试剂盒(磁珠法)  192次 英文PDF-中文PDF A-P-1050-192 点击这里给我发消息
抗人C5a/C5a desArg单克隆抗体(克隆号2942) 100ug C-HM2078  
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 100ug C-HM2022F  
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 50ug C-HM2008  
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 50ug C-HM2006  
抗小鼠TREM-2单克隆抗体(克隆号6E9) 100ug PDF  C-HM1129F 点击这里给我发消息
m6A RNA甲基化定量检测试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-9005-96  
RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-P-9003-050  
EZ RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-R5001  

表观遗传学产品全面解决方案列下:     

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1036-96  
DNA羟甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1037-96  
DNA甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 英文PDF--中文PDF A-P-1014B-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 英文PDF--中文PDF A-P-1014B-96  

推荐阅读: 

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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