Nsi I (Zsp2 I)
Origin:
Zoogloea species 2
Concentration:
5000 - 20000, u.a./ml
Recognition site:
ATGCA/T
T/ACGTA
Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 100 |
50-75 |
25-50 |
25-50 |
25-50 |
| Product |
| Cat. No. |
Size |
| SZS101S |
200 U |
| SZS101M |
1000 U |
| SZS101L |
5000 U |
  
| Product (停產) |
| Cat. No. |
Size |
| E145S |
200 U |
| E145 |
1000 U |
| E146 |
5000 U |
|
