Not I (CciN I)

Origin:
Nocardia otitidis -caviarum

Concentration:
10 u/µl

Recognition site:
GC/GGCCGC
CGCCGG/CG

Reaction buffer:
GM-buffer III, (50 mM Tris-HCl 【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA)

Storage conditions:
20 mM Tris-HCI (pH 7.4 @ 25°C), 200 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA, 50% glycerol

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation:
65°C for 20 min.

Enzyme activity in % of maximum: