Nla IV (BspL I, PspN4 I)

Origin:
Neisseria lactamica

Concentration:
1 u/µl

Recognition site:
GGN/NCC
CCN/NGG

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl (pH 7.5 @ 25°C), 200 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 500 μg/ml BSA, 50% Glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 10-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: