Hinf I

Origin:
E.coli strain that carries the cloned Hinf I gene from Haemophilus influenzae

Concentration:
20000, u.a./ml

Recognition site:
G/ANTC
CTNA/G

Reaction buffer:
GM-buffer O, (50mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum: