Hae II (BstH2 I)

Origin:
Haemophilus aegypticus

Concentration:
20 u/µl

Recognition site:
RGCGC/Y
Y/CGCGR

Reaction buffer:
GM-buffer IV,  (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl(pH 7.4 @ 25°C), 50 mM KCl, 1 mM dithiothreitol , 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol.

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 65°C.

Optimum temperature: 37°C

Inactivation 20 minutes: 80°C for 20 min.

Enzyme activity in % of maximum: