Fok I

Origin:
Flavobacterium okeanokoites

Concentration:
4 u/µl

Recognition site:
GGATG(N)₉/
CCTAC(N)₁₃/

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
10 mM Tris-HCl(pH 7.4 @ 25°C), 50 mM NaCl, 1 mM dithiothreitol, 0.1 mM EDTA, 200 μg/ml BSA, 0.1% tween 20, 50% Glycerol

Storage temperature:
-20°C

Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 20-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation: 65°C for 20 min.

Enzyme activity in % of maximum: