EcoR V
Origin:
E.coli that carries the EcoR V gene from plasmid J62 pLG74
Concentration:
20 u/µl
Recognition site:
GAT/ATC
CTA/TAG
Reaction buffer:
GM-buffer III, (50 mM Tris-HCl 【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA)
Storage conditions:
10 mM Tris-HCl (pH 7.4 @ 25°C), 50 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol.
Storage temperature:
-20°C
Ligation and recutting:
More than 95% of DNA fragments (1 μg) digested with 50-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.
Optimum temperature: 37°C
Heat inactivation: 65°C for 20 min.
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 0 |
100 |
100 |
50 |
100 |
| Product |
| Cat. No. |
Size |
| SZ035S |
800 U |
| SZ035M |
4000 U |
| SZ035L |
20000 U |
  
| Product (停產) |
| Cat. No. |
Size |
| E059S |
1000 U |
| E059 |
2000 U |
| E060 |
10000 U |
|
