BstX I
Origin:
Bacillus stearothermophilus X
Concentration:
10 u/µl
Recognition site:
CCANNNNN/NTGG
GGTN/NNNNNACC
Reaction buffer:
GM-buffer III, (50 mM Tris-HCl【pH 7.9 @ 25°C】,100 mM NaCl, 10 mM MgCl2, 100 μg/ml BSA)
*This product is NOT recommended using GM-ONE buffer
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Heat inactivation: 65°C for 20 min.
Enzyme activity in % of maximum:
| I |
II |
III |
IV |
ONE |
| 10 |
100 |
100 |
50 |
NR |
| Product |
| Cat. No. |
Size |
| SZS110S |
100 U |
| SZS110M |
500 U |
| SZS110L |
2500 U |
  
| Product (停產) |
| Cat. No. |
Size |
| E465S |
100 U |
| E465 |
500 U |
| E466 |
2500 U |
|
