BstN I (Bst2U I, PspG I)
Origin:
Bacillus stearothermophilus 2U
Concentration:
10000 - 20000, u.a./ml
Recognition site:
CC/WGG
GGW/CC
Reaction buffer:
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25℃); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After BstN I overdigestion none of DNA fragments can be ligated.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 60°C.
Optimum temperature:
60°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
| B |
G |
O |
W |
Y |
| 75-100 |
100 |
50-75 |
50-75 |
10-25 |
| Product |
| Cat. No. |
Size |
| SZS095S |
1000 U |
| SZS095M |
2000 U |
| SZS095L |
10000 U |
  
| Product (停產) |
| Cat. No. |
Size |
| E051S |
1000 U |
| E051 |
2000 U |
| E052 |
10000 U |
|
