BssH II (BseP I)
Origin:
Bacillus stearothermophilus P
Concentration:
5000, u.a./ml
Recognition site:
G/CGCGC
CGCGC/G
Reaction buffer:
GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Optimum temperature:
50°C
Inactivation 20 minutes:
under 65 °C
Enzyme activity in % of maximum:
| B |
G |
O |
W |
Y |
| 50-75 |
100 |
75-100 |
50-75 |
50-75 |
| Product |
| Cat. No. |
Size |
| SZS102S |
200 U |
| SZS102M |
1000 U |
  
| Product (停產) |
| Cat. No. |
Size |
| E181 |
200 U |
| E182 |
1000 U |
|
